PCR multiplex para identificação de isolados de Clostridium chauvoei e Clostridium septicum

Complexity of toxins from Clostridium septicum and Clostridium chauvoei.

Production of toxins by Clostridium septicum and Clostridium chauvoei has been the subject of many conflicting reports. Roux and Chamberland (1887) first noted that 3 to 4 day filtrates were antigenic; conferring on guinea pigs an immunity against infection with viable organisms. Production of a lethal toxin by C. septicum was however, demonstrated by Leclainche (1898) and Leclainche and Morel ...

Extraction de la toxine de Clostridium chauvoei a partir des corps microibens

In vitro evaluation of Clostridium septicum alpha toxoid [Avaliação in vitro de toxoide alfa de Clostridium septicum]

Aiming to investigate in vitro alternatives, a test for neutralizing antibody detection using cell culture was developed. This test was more sensitive than previous animal models, allowing for detection of substantially lower alpha toxin and anti-alpha toxin titers. Titers observed during in vivo and in vitro seroneutralization had a correlation of 99.12%, indicating that cell culture is a viab...

Novel real-time PCR assay for simultaneous detection and differentiation of Clostridium chauvoei and Clostridium septicum in clostridial myonecrosis.

A real-time PCR assay based on the 16S rRNA gene sequence was designed for differentiation of blackleg-causing Clostridium chauvoei and Clostridium septicum, a phylogenetically closely related bacterium responsible for malignant edema. In order to exclude false-negative results, an internal amplification control was included in the assay. A set of three probes, one specific for C. chauvoei, one...

Immunizalion of Cattle with Clostridium chauvoei Vaccine